pas glycogen staining kit  (Beyotime)

Journal: Frontiers in Pharmacology

Article Title: Modified gegen qinlian decoction ameliorates Veillonella parvula -exacerbated ulcerative colitis via restoration of intestinal mucosal barrier function

doi: 10.3389/fphar.2026.1803816

Figure Lengend Snippet: V. parvula exacerbates colonic mucus barrier damage in DSS-induced colitis mice, while the MGQD ameliorates this V. parvula -exacerbated damage. (A,B) AB-PAS staining in mouse colon. (C) The IF staining of mouse colon for Muc2 (red) and DAPI (blue). Data are expressed as mean ± S.D. (n = 6). #P < 0.05, ##P < 0.01, ###P < 0.001 vs. the DSV group. *P < 0.05, **P < 0.01, ***P < 0.001 vs. the DSS group.

Article Snippet: Hematoxylin and eosin (H&E) and Alcian Blue/Periodic Acid-Schiff (AB/PAS) staining kits were provided by Servicebio (Wuhan, China, Batch number:G1049).

Techniques: Staining

Journal: Frontiers in Pharmacology

Article Title: Modified gegen qinlian decoction ameliorates Veillonella parvula -exacerbated ulcerative colitis via restoration of intestinal mucosal barrier function

doi: 10.3389/fphar.2026.1803816

Figure Lengend Snippet: V. parvula exacerbates colonic barrier damage in DSS-induced colitis pseudo-germ-free mice, while the MGQD ameliorates this V. parvula -exacerbated damage. (A) The AB-PAS staining in mouse colon. (B) The IF staining of mouse colon for Muc2 (red) and DAPI (blue). (C) The Western blot of mouse colon. The data are expressed as the means ± S.D. (n = 6). *P < 0.05, **P < 0.01, ***P < 0.001 vs. the VD group.

Article Snippet: Hematoxylin and eosin (H&E) and Alcian Blue/Periodic Acid-Schiff (AB/PAS) staining kits were provided by Servicebio (Wuhan, China, Batch number:G1049).

Techniques: Staining, Western Blot

Journal: iScience

Article Title: Parabacteroides distasonis ameliorates ovariectomy-induced bone loss by regulating the gut microbiota and Th17/Treg balance

doi: 10.1016/j.isci.2026.115345

Figure Lengend Snippet: LPD improves colonic pathology (A) Serum LPS levels ( n = 8). (B) Morphological research measures include the number of goblet cells in the gland ( n = 3). (C) Representative pictures of HE staining, Scale bars, 100 μm. (D) Representative images of Ab-PAS staining, Scale bars, 100 μm. Data are presented as mean ± SEM ( n = 3–8 rats per group). For histological analyses, multiple sections and fields were averaged within each animal prior to statistical analysis. Statistical significance was determined by one-way ANOVA followed by Tukey’s post hoc test. ∗∗∗ p < 0.001.

Article Snippet: Ab-PAS Stain Kit , Servicebio , Cat. No. G1049.

Techniques: Staining

Journal: STAR Protocols

Article Title: Protocol for developing a mucus-producing gut-on-a-chip model from Caco-2 and HT29-MTX-E12 cells

doi: 10.1016/j.xpro.2025.104321

Figure Lengend Snippet: Mucus production, barrier integrity and cell viability evaluation for GoC characterization (A) Mucus production in co-culture 9:1 and 7:3 with Alcian blue/Periodic Acid Schiff’s staining. (B) Barrier integrity with LY (20 μg/ml) for co-culture 9:1 and 7:3 day 2, 4, 6, 8; blank - empty chip (Data are represented as mean ± SD. Test: One-way ANOVA t test, p < 0.01 “∗∗”, p < 0.0001 “∗∗∗∗”, n = 4). (C) Barrier integrity with LY (20 μg/ml) for co-culture 9:1 and 7:3 day 2, 4, 6, 8 (Data are represented as mean ± SD. Test: Two-way ANOVA t test, p < 0.05 “∗”, non-significant “ns”, n = 4). (D) Cell viability with CCK-8 cell viability kit for co-culture 9:1, 7:3 and viability control for co-culture 7:3 (0.25% Triton X-100 added in CM in flow for 24 hours at 2 μL/min) (Data are represented as mean ± SD. Test: One-way ANOVA t test, non-significant “ns”, p < 0.0001 “∗∗∗∗”, n = 4). (E) Variance using barrier integrity test with LY (20 μg/ml) for co-culture 7:3 day 2, 4, 6, 8 (Data are represented as mean ± SD. Test: Two-way ANOVA t test, p < 0.05 “∗”, p < 0.0001 “∗∗∗∗”, non-significant “ns”, n = 12–3 independent chips). (F) Variance using cell viability test with CCK-8 cell viability kit for co-culture 7:3 on day 8 in three chips independently No1, No2, No3 (n = 4) and all of them combined No1 + No2 + No3 (n = 12) (Data are represented as mean ± SD. Test: One-way ANOVA t test, non-significant “ns”).

Article Snippet: EprediaTM Alcian Blue/PAS Special Stain Kit , Fisher Scientific , 23-900-664.

Techniques: Co-Culture Assay, Staining, CCK-8 Assay, Control